Fetal Bovine serum, dialysed

Fetal Bovine serum, dialysed

Fetal Bovine Serum: 12-14 kD dialysate, heat-inactivated summary

Dialyzed fetal bovine serum is used in cell culture systems that require a more defined small molecule environment. Dialysis reduces the concentration of low molecular weight components such as nucleotides, amino acids, hormones, salts, and various small proteins in serum.

Key benefits

  • Dialysis performed with a 12-14 kDa cutoff membrane
  • Process controlled by glucose monitoring
  • Monitored during dialysis to prevent precipitation and inactivation of peptides in the FBS
  • Excellent cell growth characteristics and low endotoxin
  • Mexico / Central America FBS approved by USDA
  • Manufactured in our ISO 9001: 2015 certified facilities
  • Previously sold under the Atlanta Biologicals brand

Why use dialyzed, heat-inactivated fetal calf serum?

Preselected fetal bovine serum, also known as fetal calf serum (FCS), is dialyzed against physiological saline using a 12,000 to 14,000 Dalton cutoff membrane. The process is controlled by precisely controlling the glucose concentration throughout the procedure. Extensive dialysis is not performed to avoid precipitation and inactivation of serum peptides. All batches of 12-14 kD dialyzed FBS have manufactured in our ISO 9001: 2015 certified facility.

Heat inactivation: serum is inactivated by raising the temperature to 56 ° C for 30 minutes under controlled conditions. The goal of heat inactivation is to destroy complement activity in serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from serum is not necessary for most cell cultures but may be necessary for cultures sensitive to complement activity.

Since heat inactivation of serum can, to some extent, decrease the performance properties of serum growth, this procedure should only be performed if it is truly necessary for optimal cell growth. Researchers must evaluate the applicability of heat inactivation with respect to their own application. If heat inactivation is required, the process must be carefully controlled to avoid further formation of crystalline and flocculating precipitates, gelation of serum proteins, and excessive loss of growth performance.

Collection, processing, and testing of R&D systems FBS

Collection and Processing: R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures that the product is traceable to the date and location of the collection. Our FBS manufacturing adheres to closed system collection, positive pressure processing, HEPA filtered clean rooms, and 0.1 um triple filtration to remove contaminants. Tight process control from harvest to processing enables us to provide a stable, consistent and traceable supply of quality serum through global fluctuations in serum availability and regulatory requirements.

Virus tests: fetal bovine serum: 12-14 kD dialysates are tested for adventitious viruses, such as bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBRV), parainfluenza-3 virus (PI-3V), Bluetongue Virus (BTV)), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5 ), Reovirus, and rabies virus.

Biochemical Testing: FBS R&D systems are biochemical profiled including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium, and potassium. Please refer to the product insert for a complete list of biochemical profiling components, which may vary between grades.

Chemical Analysis: Each batch of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method), and microbiological contamination (using the methods recommended by the US Pharmacopeia).

 

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