This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with the human novel. coronavirus nucleoprotein. Samples are pipetted into the wells with anti-human. IgG-conjugated horseradish peroxidase (HRP).
Any specific antibody for the antigen present will bind to the precoated antigen. After washing to remove any unbound reagent, a substrate solution is added to the wells and stained develops in proportion to the amount of human IgG antibody against SARS-CoV-2 N linked in the initial step. Color development stops and the intensity of the color is measured.
SPECIFICITY
This assay has high sensitivity and excellent specificity for the detection of humans. IgG antibody to SARS-CoV-2 N. No significant interference or cross-reactivity between human SARS-CoV-2 N IgG antibody and analogs.
Note: Limited by current skills and knowledge, it is impossible for us to complete the detection of cross-reactivity between human SARS-CoV-2 N IgG antibody and all analogs, therefore, may still cross-react.
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess
