This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with the human novel. coronavirus nucleoprotein / S1 peak glycoprotein. Samples are pipetted into the wells with horseradish peroxidase (HRP) conjugated to anti-human IgG.
Some Antibodies specific for the antigen present will bind to the pre-coated antigen. After a wash to remove any unbound reagent, a substrate solution is added to the wells and the color develops in proportion to the number of humans SARS-CoV-2 N / S1 IgG antibody bound in the initial step. The development of color stops and the intensity of the color is measured.
This assay has high sensitivity and excellent specificity for the detection of humans. IgG antibody against SARS-CoV-2 N / S1. No significant interference or cross-reactivity between the human IgG antibody SARS-CoV-2 N / S1 and analogs it was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete detection of cross-reactivity between SARS-CoV-2 N / S1 human IgG antibody and all analogs therefore cross-reaction may still exist.
Dilute the serum or plasma samples with Sample Diluent(1:100) before a test. The suggested 100-fold dilution can be achieved by adding a 10μl sample to 40μl of Sample Diluent. Complete the 100-fold dilution by adding 15μl of this solution to 285μl of Sample Diluent.