The spike glycoprotein comprises two functional subunits responsible for host cell receptor binding (S1 subunit) and fusion of the viral and cell membranes (S2 subunit). For many coronaviruses (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation.
The distal S1 subunit comprises the receptor-binding domains and contributes to the stabilization of the prefusion state of the membrane-anchored S2 subunit containing the fusion machinery. S is further cleaved by host proteases at the so-called S2 ‘site located immediately upstream of the fusion peptide in all CoVs.
- Target name Novel coronavirus RBD spike glycoprotein (SARS-CoV-2 S1 RBD)
- Alternative names 2019 novel coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human coronavirus 2019;
- SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; RBD peak glycoprotein; Spike Protein RBD
- Abbreviation SARS-CoV-2 S1 RBD
- Uniprot No. P0DTC2
- Sample types serum, plasma, swabs
- Detection range 1.25 ng / mL-80 ng / mL
- Sensitivity 0.31 ng / mL
- Test time 1-5 h
- Sample volume 50-100ul
- Detection wavelength 450 nm
- Infectious Diseases research area
- Quantitative test principle
- Measuring sandwich
Intra-assay precision (precision within an assay): CV% <8%Three samples of known concentration were run twenty times on a plate for evaluation.Inter-assay precision (precision between assays): CV% <10%Three samples of known concentration were analyzed in twenty tests for evaluation.
This cleavage has been proposed to activate the membrane fusion protein through extensive irreversible conformational changes. However, different CoVs use different domains within the S1 subunit to recognize a variety of entry and binding receptors, depending on the viral species. The endemic human coronaviruses OC43 and HKU1 bind via their SA domain to the 5-N-acetyl-9-O-acetyl Asia sides found in glycoproteins and glycolipids on the surface of the host cell to allow entry to susceptible cells.
MERS-CoV S uses domain A to recognize non-acetylated sial side-binding receptors, which likely promote the subsequent binding of domain B to the input receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses ( SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) through SB to enter target cells.